It happened on U.S. Highway 141 and County Highway P.
Police say the motorcycle was headed southbound on Highway 141 when it struck by a car coming off of County P.
The motorcycle was driven by a 52-year-old Kimberly man. His 52-year-old wife was his passenger.
Police say the female passenger of the motorcycle was seriously injured. She and her husband were transported to a local hospital in Marinette.
The woman was later flown by Eagle III to a Green Bay Hospital.
The driver of the car, a 19-year-old Coleman man, and his passenger, a 16-year-old female from Crivitz, suffered minor injuries.
Police say drugs and alcohol do not appear to be factors in this crash. The incident remains under investigation.