Coach Lisa Joling says in her complaint that male coaches who allowed more egregious songs to be played in locker rooms or at school functions faced no penalties.
Joling tells News-Herald Media that her firing has hurt her reputation and the private business she owns.
"Blurred Lines" topped the Billboard 100 chart for 12 weeks, but critics say its sexually suggestive lyrics perpetuate misogynistic attitudes toward women.
The dance team performed to the song at the first home football game.
Marshfield School District Superintendent Peg Geegan says the district is reviewing Joling's complaint.