Officials say a passerby saw smoke coming from the building, and called 911 around 9:24 p.m.
The fire was contained to the office building at Ridgeway Trailer Co. on the 3100 block of S. Ridge Rd.
According to the company's website, it is a semi-trailer repair shop.
Fire officials say they were able to stop the blaze from spreading to the garage where the trailers are stored.
Authorities say no one was hurt.
Multiple departments were called in to help, including; Ashwaubenon, Howard, Hobart, Lawrence, De Pere and Green Bay.